'A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity'. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Southern blotting is used for testing to ensure that a particular section of DNA of. Hybridization probe size control: optimized 'oligolabelling'. Southern blotting combines agarose gel electrophoresis for size. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Expression and regulation of the murine serum amyloid A (SAA) gene in extrahepatic sites. In dilution experiments of CML cells with normal cells, a linear relationship could be demonstrated between the relative intensity of the autoradiograph band corresponding. Overexpression of the epidermal growth factor receptor in human pancreatic cancer is associated with concomitant increases in the levels of epidermal growth factor and transforming growth factor alpha. We have assessed the feasibility of estimating the relative proportion of leukemic cells present in a sample by densitometric analysis of autoradiographs of Southern blots. Lock the cassette and place it at -80 ☌ overnight. Carry the cassette to the darkroom and place the X-ray film over the blot. The Western blot protocol begins with the separation of larger molecules via electrophoresis. Place the blot in a film cassette lined with new saran wrap and carefully wrap the blot ensuring no air bubbles are trapped between the blot and the wrap. Western blotting or immunoblotting can pinpoint one or more specific protein antibodies from a sample via a standard protocol. Enhanced expression of transforming growth factor beta isoforms in pancreatic cancer correlates with decreased survival. The Western blot test detects proteins of the immune system and has been used since the late 1970s. Enhanced expression of the type II transforming growth factor beta receptor in human pancreatic cancer cells without alteration of type III receptor expression. Meta-analysis of microarray data on pancreatic cancer defines a set of commonly dysregulated genes. Identification of disease-specific genes in chronic pancreatitis using DNA array technology. Microarray-based identification of differentially expressed growth- and metastasis-associated genes in pancreatic cancer. The use of reverse transcriptase-polymerase chain reaction (RT-PCR) to investigate specific gene expression in multidrug-resistant cells. Pancreatic cancer: from bench to 5-year survival. Molecular pathogenesis of pancreatic cancer: advances and challenges. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
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